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KMID : 0613820130230020267
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Journal of Life Science
2013 Volume.23 No. 2 p.267 ~ p.272
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Micrografting and Heat Treatment Combination for Eliminating Virus of CTV-infected Citrus
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Chae Chi-Won
Yun Su-Hyun
Park Jae-Ho
Hyun Jae-Wook
Koh Sang-Wook
Lee Dong-Hoon
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Abstract
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This study was conducted to eliminate viruses from citrus-infected plants using micrografting and thermotherapy. Six citrus cultivars including a ’Setoka’ hybrid were used as plant sources. The TAS-ELISA technique demonstrated that several plants were CTV positive. However, no CTV symptoms were detected in plants obtained from shoots and treated at a high temperature of 40℃ during the day and night and micrografted for two weeks with old trifoliate orange rootstock in vitro. Indexing of CTV, SDV, and CTLV for RT-PCR analysis of the eleven citrus seedlings, including ’Setoka’, ’Samdajosang’, ’Pungkwang’, ’Shiranuhi’, and ’Ehimekashi dai28go’ was virus free following the micrografting and thermal therapy.
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KEYWORD
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Citrus, CTV, virus free plant, RT-PCR, TAS-ELISA
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